Crystal Structure of the Putidaredoxin Reductase·Putidaredoxin Electron Transfer Complex
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منابع مشابه
Crystal Structure of the Putidaredoxin Reductase- Putidaredoxin Electron Transfer Complex
CRYSTAL STRUCTURE OF THE PUTIDAREDOXIN REDUCTASEPUTIDAREDOXIN ELECTRON TRANSFER COMPLEX Irina F. Sevrioukova, Thomas L. Poulos, and Inna Y. Churbanova⊥, From the Departments of Molecular Biology and Biochemistry, Chemistry and Pharmaceutical Sciences, University of California, Irvine, California 92697-3900 Running title: Crystal structure of the Pdr-Pdx complex Address correspondence to: Irina ...
متن کاملCrystal structure of the putidaredoxin reductase x putidaredoxin electron transfer complex.
In the camphor monooxygenase system from Pseudomonas putida, the [2Fe-2S]-containing putidaredoxin (Pdx) shuttles electrons between the NADH-dependent putidaredoxin reductase (Pdr) and cytochrome P450(cam). The mechanism of the Pdr.Pdx redox couple has been investigated by a variety of techniques. One of the exceptions is x-ray crystallography as the native partners associate weakly and resist ...
متن کاملThe putidaredoxin reductase-putidaredoxin electron transfer complex: theoretical and experimental studies.
Interaction and electron transfer between putidaredoxin reductase (Pdr) and putidaredoxin (Pdx) from Pseudomonas putida was studied by molecular modeling, mutagenesis, and stopped flow techniques. Based on the crystal structures of Pdr and Pdx, a complex between the proteins was generated using computer graphics methods. In the model, Pdx is docked above the isoalloxazine ring of FAD of Pdr wit...
متن کاملThe Putidaredoxin Reductase-putidaredoxin Electron Transfer Complex: Theoretical and Experimental Study*
THE PUTIDAREDOXIN REDUCTASE-PUTIDAREDOXIN ELECTRON TRANSFER COMPLEX: THEORETICAL AND EXPERIMENTAL STUDY* Vadim Yu. Kuznetsov, Emek Blair, Patrick J. Farmer, Thomas L. Poulos, Amanda Pifferitti, and Irina F. Sevrioukova From the Departments of Molecular Biology and Biochemistry, Chemistry, and Physiology and Biophysics and the Program in Macromolecular Structure, University of California, Irvine...
متن کاملCrystal structure of the electron transfer complex rubredoxin rubredoxin reductase of Pseudomonas aeruginosa.
Crude oil spills represent a major ecological threat because of the chemical inertness of the constituent n-alkanes. The Gram-negative bacterium Pseudomonas aeruginosa is one of the few bacterial species able to metabolize such compounds. Three chromosomal genes, rubB, rubA1, and rubA2 coding for an NAD(P)H:rubredoxin reductase (RdxR) and two rubredoxins (Rdxs) are indispensable for this abilit...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2010
ISSN: 0021-9258
DOI: 10.1074/jbc.m110.104968